CHRM2 Antibody

• Description:

  The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)

• UniProt:

  P08172

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  A recombinant human protein was used as the immunogen for the CHRM2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1424CT461.78.60

• Applications:

  IHC-P, WB, IF, FACS

• Purity:

  Protein G affinity

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CHRM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CHRM2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CHRM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the CHRM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC testing of FFPE human brain tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.