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Vinculin Antibody

CAT: 0800-F54324-0.2MLSize: 0.2 mLDry Ice: NoHazardous: No
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CAT#:0800-F54324-0.2MLSize:0.2 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity) .
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
Q64727
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 903-937 from the human protein was used as the immunogen for the Vinculin antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
Aliquot the Vinculin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

UniProtKB · Q64727

Vinculin

VINC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q64727
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Vcl
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q8BP32, Q8BS46, Q922C5, Q922D9
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationLipoproteinPalmitatePhosphoprotein

Molecular function

Actin-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCell projectionCytoplasmCytoskeletonMembrane

Domain

Repeat
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