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Glucagon Antibody

CAT: 0800-F54303-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54303-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glucagon is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
UniProt
P01275
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 119-148 from the human protein were used as the immunogen for the Glucagon antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Glucagon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Glucagon antibody is available for research use only.
Storage Conditions
Aliquot the Glucagon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Glucagon antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC testing of FFPE human pancreas tissue with Glucagon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Product Datasheet
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