GARS Antibody

• Description :

  Glycine--tRNA ligase catalyzes the attachment of glycine to tRNA (Gly) . Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 1:1000-2000, FACS (intracellular) : 1:25

• UniProt :

  P41250

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A human recombinant protein of amino acids 15-305 was used as the immunogen for the GARS antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  1641CT837.23.96

• Applications :

  WB

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the GARS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This GARS antibody is available for research use only.

• Storage Conditions :

  Aliquot the GARS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the GARS antibody may be required due to differences in protocols and substrate sensitivity.

• Image Legend :

  Western blot testing of human 1) Jurkat, 2) Ramos and 3) HT-1080 cell lysate with GARS antibody at 1:2000. Predicted molecular weight: 83 kDa.