CD25 Antibody

• Description:

  The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

• Specifications:

  Western blot: 1:100-1:800

• UniProt:

  P01589

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A purified His-tagged recombinant protein fragment was used to produced this CD25 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  7G7B6

• Applications:

  WB, ELISA

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  In ascites with 0.09% sodium azide

• Reconstitution:

  Aliquot the CD25 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD25 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD25 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In ascites with 0.09% sodium azide

• Applications Notes:

  Titration of the CD25 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  CD25 antibody western blot analysis in Jurkat lysate