RICTOR Antibody

• Description :

  Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

• Specifications :

  Western blot: 1:2000

• UniProt :

  Q6R327

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This RICTOR antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1068-1102 amino acids from the Central region of human RICTOR.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the RICTOR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This RICTOR antibody is available for research use only.

• Storage Conditions :

  Aliquot the RICTOR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the RICTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of RICTOR antibody at 1:2000 dilution + HepG2 lysate; Predicted molecular weight ~200 kDa.