BMI1 Antibody

• Description:

  Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

• Specifications:

  Western blot: 1:1000-2000

• UniProt:

  P35226

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This BMI1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 230-265 amino acids from the C-terminal region of human BMI1.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the BMI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This BMI1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the BMI1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the BMI1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of BMI1 antibody at 1:2000 dilution. Lane 1: SW480 lysate; 2: HeLa lysate; Predicted molecular weight: 37-43 kDa.