FBXW7 Antibody

• Description:

  Substrate recognition component of an SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. [UniProt]

• Specifications:

  Western blot: 1:2000

• UniProt:

  Q969H0

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This FBXW7 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 133-167 amino acids from the N-terminal region of human FBXW7.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the FBXW7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This FBXW7 antibody is available for research use only.

• Storage Conditions:

  Aliquot the FBXW7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the FBXW7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of FBXW7 antibody at 1:2000 dilution + HeLa lysate; Predicted molecular weight: 80 kDa (alpha), 70 kDa (beta) .