SMAD2 Antibody

• Description:

  SMAD2 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q15796

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This SMAD2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 106-140 amino acids from the N-terminal region of human SMAD2.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SMAD2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of SMAD2 antibody at 1:1000 dilution + NIH3T3 lysate; Predicted molecular weight: 52~60 kDa.