EpCAM Antibody

• Description :

  May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity) .

• CAS Number :

  9007-83-4

• Specifications :

  Flow Cytometry: 1:25, Western blot: 1:1000

• UniProt :

  Q99JW5

• Host :

  Rabbit

• Reactivity :

  Mouse, Human

• Immunogen :

  This mouse Epcam antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  FACS, WB, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution :

  Aliquot the EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This EpCAM antibody is available for research use only.

• Storage Conditions :

  Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the EpCAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Rat

• Image Legend :

  Flow cytometric analysis of HepG2 cells using Epcam antibody (green) compared to an isotype control of rabbit IgG (blue) ; Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.