SMAD2 Antibody

• Description :

  SMAD2 is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. [UniProt]

• Specifications :

  Western blot: 1:500-1:1000

• UniProt :

  Q62432

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  This SMAD2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids of mouse Smad2.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Store at 4oC for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This SMAD2 antibody is available for research use only.

• Storage Conditions :

  Store at 4°C for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Bovine, Pig, Chicken

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of lysate from (1) HeLa, (2) mouse NIH3T3, (3) mouse C2C12, and (4) rat PC-12 cell line using SMAD2 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.