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SMAD2 Antibody

CAT: 0800-F52960-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F52960-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SMAD2 is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:1000
UniProt
Q62432
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
This SMAD2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids of mouse Smad2.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Store at 4oC for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD2 antibody is available for research use only.
Storage Conditions
Store at 4°C for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Pig, Chicken
Image Legend
Western blot analysis of lysate from (1) HeLa, (2) mouse NIH3T3, (3) mouse C2C12, and (4) rat PC-12 cell line using SMAD2 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.
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