Egfr Antibody

• Description :

  Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

• Specifications :

  Western blot: 1:1000

• UniProt :

  Q01279

• Host :

  Rabbit

• Reactivity :

  Mouse

• Immunogen :

  This mouse Egfr antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1116-1130 amino acids from the human region of mouse Egfr.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the Egfr antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This Egfr antibody is available for research use only.

• Storage Conditions :

  Aliquot the Egfr antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the Egfr antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, membranous

• Image Legend :

  Western blot analysis of lysate from mouse liver tissue using Egfr antibody diluted at 1:1000. Expected molecular weight: ~134/170 kDa (unmodified/glycosylated) .