SP1 Antibody / Specificity Protein 1

• Description:

  Specificity Protein 1/SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation) . Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. [UniProt]

• Specifications:

  Flow Cytometry: 1:25, Immunofluorescence: 1:25, Western blot: 1:1000

• UniProt:

  P08047

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  This SP1 antibody was produced from a mouse immunized with recombinant Specificity Protein 1.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1326CT463.109.176

• Applications:

  WB, FACS, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the SP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SP1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the SP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the SP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from human (1) HeLa and (2) Ramos cell line using SP1 antibody at 1:1000. Reported molecular weight: 81-95 kDa.