MGMT Antibody

• Description:

  Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

• Specifications:

  IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:1000

• UniProt:

  P16455

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified His-tagged protein was used to produced this monoclonal MGMT antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  888CT22.2.4

• Applications:

  IHC, IF, WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MGMT antibody is available for research use only.

• Storage Conditions:

  Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunohistochemical analysis of paraffin-embedded human small intestine using MGMT antibody at 1:25 dilution.