MGMT Antibody

• Description :

  Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

• Specifications :

  IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:1000

• UniProt :

  P16455

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Purified His-tagged protein was used to produced this monoclonal MGMT antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  888CT22.2.4

• Applications :

  IHC, IF, WB, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This MGMT antibody is available for research use only.

• Storage Conditions :

  Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Immunohistochemical analysis of paraffin-embedded human small intestine using MGMT antibody at 1:25 dilution.