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MGMT Antibody

CAT: 0800-F52339-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F52339-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:1000
UniProt
P16455
Host
Mouse
Reactivity
Human
Immunogen
Purified His-tagged protein was used to produced this monoclonal MGMT antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
888CT22.2.4
Applications
IHC, IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MGMT antibody is available for research use only.
Storage Conditions
Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunohistochemical analysis of paraffin-embedded human small intestine using MGMT antibody at 1:25 dilution.

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