IkBa Antibody

• Description:

  Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

• Specifications:

  Western blot: 1:1000

• UniProt:

  P25963

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified His-tagged protein was used to produced this monoclonal IkB alpha antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1121CT8.6.1

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the IkBa antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This IkBa antibody is available for research use only.

• Storage Conditions:

  Aliquot the IkBa antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the IkBa antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IkBa antibody western blot analysis in human HeLa and mouse NIH3T3 cell lysate.