AIM2 Antibody

• Description:

  Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity) . Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. [UniProt]

• Specifications:

  Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt:

  O14862

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 1-50 from the human protein was used as the immunogen for this AIM2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, FACS, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the AIM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This AIM2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the AIM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the AIM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membrane, nuclear

• Image Legend:

  Western blot analysis of AIM2 antibody and 1) MCF-7, 2) HL-60, 3) K562 and 4) HeLa lysate. Predicted molecular weight 40-45 kDa.