HuR Antibody

• Description:

  ELAVL1/HuR is involved in 3'-UTR ARE-mediated MYC stabilization. It binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt:

  Q15717

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HuR antibody was produced from rabbits immunized with human ELAVL1/HuR recombinant protein.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, WB, IHC, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the HuR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This HuR antibody is available for research use only.

• Storage Conditions:

  Aliquot the HuR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the HuR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of HuR antibody and HeLa, Jurkat, CEM, Ramos lysate.