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BMI1 Antibody

CAT: 0800-F51332-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F51332-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P35226
Host
Rabbit
Reactivity
Human
Immunogen
This BMI1 antibody was produced from rabbits immunized with BMI1 recombinant protein.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, IHC, IF, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the BMI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This BMI1 antibody is available for research use only.
Storage Conditions
Aliquot the BMI1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the BMI1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Confocal immunofluorescent analysis of BMI1 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) .

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