PERK Antibody

• Description :

  PERK, a member of the GCN2 subfamily of Ser/Thr protein kinases, phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It likely serves as a critical effector of unfolded protein response (UPR) -induced G1 growth arrest due to the loss of cyclin D1 Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction Expression of this Type I membrane protein is ubiquitous, with highest levels seen in secretory tissues. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt :

  Q9NZJ5

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 148-175 from the human protein was used as the immunogen for this PERK antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the PERK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This PERK antibody is available for research use only.

• Storage Conditions :

  Aliquot the PERK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the PERK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Location :

  Cytoplasmic, ER membrane

• Image Legend :

  Western blot analysis of PERK antibody and 293 lysate. Predicted molecular weight ~125 kDa.