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DHFR Antibody

CAT: 0800-F50355-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F50355-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
P00375
Host
Rabbit
Reactivity
Mouse
Immunogen
A portion of amino acids 135-164 from mouse Dihydrofolate reductase was used as the immunogen for this DHFR antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This DHFR antibody is available for research use only.
Storage Conditions
Aliquot the DHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of DHFR antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the DHFR gene. Predicted molecular weight ~22 kDa.

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