SMAD2 Antibody

• Description :

  Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

• Specifications :

  Immunofluorescence: 1:100, Western blot: 1:1000, Flow Cytometry: 1:10-1:50

• UniProt :

  Q15796

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  A portion of amino acids 97-125 from the human protein was used as the immunogen for this SMAD2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  IF, WB, FACS, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This SMAD2 antibody is available for research use only.

• Storage Conditions :

  Aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Rat, Bovine, Pig, Chicken, Zebrafish, Drosophila

• CAS Number :

  9007-83-4

• Image Legend :

  Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.