CD28 Antibody

• Description:

  CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.

• Specifications:

  Western blot: 1:1000, Immunofluorescence: 1:10-1:50

• UniProt:

  P10747

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 182-208 from the human protein was used as the immunogen for this CD28 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CD28 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD28 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD28 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CD28 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Rabbit

• CAS Number:

  9007-83-4

• Image Legend:

  Confocal immunofluorescent analysis of CD28 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .