PAX6 Antibody

• Description:

  PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt:

  P26367

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 183-210 from the human protein was used as the immunogen for this PAX6 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, IF, FACS, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Store at 4oC for up to one month. For long term, aliquot the PAX6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PAX6 antibody is available for research use only.

• Storage Conditions:

  Store at 4°C for up to one month. For long term, aliquot the PAX6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the PAX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat, Bovine, Xenopus

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  PAX6 antibody western blot analysis in U251 lysate. Predicted molecular weight ~48kDa.