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TBP Antibody

CAT: 0800-F49783-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F49783-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50
UniProt
P20226
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 301-330 from the human protein was used as the immunogen for this TBP antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This TBP antibody is available for research use only.
Storage Conditions
Aliquot the TBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Chicken, Mouse, Xenopus
Image Legend
Western blot analysis of TBP antibody and HL-60 lysate. Observed molecular weight: 35-43 kDa.

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