VDAC Antibody

• Description:

  VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt:

  P21796

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 1-30 from human VDAC1 was used as the immunogen for this VDAC antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the VDAC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This VDAC antibody is available for research use only.

• Storage Conditions:

  Aliquot the VDAC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the VDAC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Bovine, Pig, Rabbit, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of VDAC antibody and 1) human A375 cell line and 2) mouse heart tissue lysate. Predicted molecular weight 30~35 kDa.