PPARG Antibody / PPAR gamma

• Description:

  PPARG is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity) . [UniProt]

• Specifications:

  Western blot: 1:1000, Flow Cytometry: 1:10-1:50

• UniProt:

  P37231

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 1-30 from the human protein was used as the immunogen for this PPARG antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, FACS, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the PPARG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PPARG antibody is available for research use only.

• Storage Conditions:

  Aliquot the PPARG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the PPARG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  PPARG antibody flow cytometric analysis of human HepG2 cells (green) compared to a negative control (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.