Phospho-TSC2 Antibody (pS1096)

• Description:

  In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity) . Specifically stimulates the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. Suggesting a possible mechanism for its role in regulating cellular growth (By similarity) .

• Specifications:

  Dot blot: 1:500

• UniProt:

  Q61037

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This phospho-TSC2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1096 of mouse TSC2.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  DB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the phospho-TSC2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This phospho-TSC2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the phospho-TSC2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the phospho-TSC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Dot blot analysis of phospho-TSC2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.