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Phospho-Bad Antibody (pS99)

CAT: 0800-F48369-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F48369-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X (L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:200, Dot blot: 1:500, IHC (Paraffin) : 1:50-1:100
UniProt
Q92934
Host
Rabbit
Reactivity
Human
Immunogen
This phospho-Bad antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS99 of human Bad.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, DB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-Bad antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-Bad antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Bad antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
Fluorescent confocal image of HeLa cells stained with phospho-Bad antibody at 1:200. Note the highly specific localization of phos/nonphos-Bad, predominantly to the cytoplasm.

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