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ATG12 Antibody

CAT: 0800-F46258-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F46258-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . APG12L/ATG12 is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:200, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
O94817
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this ATG12 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ATG12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATG12 antibody is available for research use only.
Storage Conditions
Aliquot the ATG12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Fluorescent image of chloroquine-treated U251 cells stained with ATG12 antibody. Alexa Fluor 488 conjugated secondary was used. ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm.

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