Alkaline Phosphatase Antibody / ALPL (tissue-nonspecific)

• Description:

  There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific) . The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

• Specifications:

  Western blot: 1:1000, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

• UniProt:

  P05186

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 217-246 from human ALPL was used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IHC, WB, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Alkaline Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Alkaline Phosphatase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Alkaline Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic, secreted

• Image Legend:

  Western blot analysis of Alkaline Phosphatase antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the ALPL gene. Predicted molecular weight ~57 kDa, but can be observed at up to ~75 kDa due to glycosylation.