TERT Antibody

• Description :

  Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.

• Specifications :

  Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:200

• UniProt :

  O14746

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 627-656 from the human protein was used as the immunogen for this TERT antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, FACS, IF, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the TERT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This TERT antibody is available for research use only.

• Storage Conditions :

  Aliquot the TERT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the TERT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Fluorescent confocal image of HeLa cell stained with TERT antibody at 1:25. Immunoreactivity is localized to the nucleus strongly and cytoplasm weakly.