AR Antibody

• Description:

  The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease) . Mutations in this gene are also associated with complete androgen insensitivity (CAIS) . Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq].

• Specifications:

  Western blot: 1:1000

• UniProt:

  P10275

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 282-309 from the human protein was used as the immunogen for this AR antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the AR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This AR antibody is available for research use only.

• Storage Conditions:

  Aliquot the AR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the AR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Primate

• CAS Number:

  9007-83-4

• Image Legend:

  AR antibody western blot analysis in (1) NCI-H292, and (1) NCI-H460 lysate. Predicted molecular weight ~99kDa.