SUMO2/3 Antibody

• Description:

  SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

• Specifications:

  Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

• UniProt:

  P55854

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 53-84 from human SUMO3 was used as the immunogen for this SUMO2/3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, IF, WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the SUMO2/3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SUMO2/3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the SUMO2/3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the SUMO2/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Hamster, Xenopus

• CAS Number:

  9007-83-4

• Image Legend:

  SUMO2/3 antibody western blot analysis in (1) 293 and (2) HeLa lysate