SUMO1 Antibody

• Description:

  Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt:

  P63165

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SUMO1 antibody was produced from rabbits immunized with human SUMO1 recombinant protein.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the SUMO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SUMO1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the SUMO1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the SUMO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of SUMO1 antibody and MCF-7 lysate. Predicted molecular weight: 12-15 kDa