MeCP2 Antibody

• Description :

  MeCP2 is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) -containing DNA, with a preference for 5-methylcytosine (5mC) . [UniProt]

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt :

  P51608

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 58-87 from the human protein was used as the immunogen for this MeCP2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, FACS, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the MeCP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This MeCP2 antibody is available for research use only.

• Storage Conditions :

  Aliquot the MeCP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Mouse, Rat

• CAS Number :

  9007-83-4

• Image Legend :

  MeCP2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.