PHOX2B Antibody

• Description:

  The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq].

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:1000

• UniProt:

  Q99453

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  A portion of amino acids 104-130 from the human protein was used as the immunogen for this PHOX2B antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the PHOX2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PHOX2B antibody is available for research use only.

• Storage Conditions:

  Aliquot the PHOX2B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the PHOX2B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Human, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Fluorescent image of U251 cell stained with PHOX2B antibody. Alexa Fluor 488 conjugated secondary (green) was used. Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin. PHOX2B immunoreactivity is localized to the nucleus.