KEAP1 Antibody

• Description:

  KEAP1 acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome [UniProt]

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt:

  Q14145

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 422-449 from the human protein was used as the immunogen for this KEAP1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Store at 4oC for up to one month. For long term, aliquot the KEAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This KEAP1 antibody is available for research use only.

• Storage Conditions:

  Store at 4°C for up to one month. For long term, aliquot the KEAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the KEAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Pig

• CAS Number:

  9007-83-4

• Image Legend:

  KEAP1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.