IKK beta Antibody

• Description:

  NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .

• Specifications:

  Western blot: 1:100-1:500

• UniProt:

  O14920

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  This IKK beta antibody was raised using purified His-tagged recombinant full length human IKKb.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  62AT992

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the IKK beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This IKK beta antibody is available for research use only.

• Storage Conditions:

  Aliquot the IKK beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the IKK beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  The IKK beta antibody used in western blot with SK-BR3 cell lysate. Predicted molecular weight ~87 kDa.