Anti-Androgen Receptor Antibody

• Description:

  Androgen receptor (ANDR) has 3 major functional domains: the N-terminal domain, DNA-binding domain, and an androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The gene for this protein contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of the protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease) . Mutations are also associated with complete androgen insensitivity (CAIS) . PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor; sumoylation of AR represses AR-dependent transcription.

• Specifications:

  Western blot: 1:100-1:250

• UniProt:

  P10275

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified His-tagged protein fragment was used to produced this monoclonal anti-Androgen Receptor antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgA

• Clone:

  549CT16.1.4

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the anti-Androgen Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This anti-Androgen Receptor antibody is available for research use only.

• Storage Conditions:

  Aliquot the anti-Androgen Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the anti-Androgen Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Anti-Androgen Receptor antibody western blot analysis in NCI-H292 lysate. Predicted molecular weight ~99kDa.