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RIZ1 Antibody

CAT: 0800-F40202-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F40202-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, ChIP: 1:100
UniProt
Q13029
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This RIZ1 antibody is specific and does not recognize the beta isoform (RIZ2) .
Clonality
Monoclonal
Isotype
IgG1
Clone
33AT1045
Applications
CHIP, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the RIZ1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This RIZ1 antibody is available for research use only.
Storage Conditions
Aliquot the RIZ1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the RIZ1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
ChIP analysis: soluble chromatin was prepared from MCF7 cells treated/untreated with E2 for 45 min. Immunoprecipitation was performed with RIZ1 antibody. DNA extractions amplified using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.

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