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PRKR Antibody

CAT: 0800-F40175-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F40175-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR. E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, which provides one mechanism by which HCV may circumvent the antiviral effect of interferon.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
P19525
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the PRKR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PRKR antibody is available for research use only.
Storage Conditions
Aliquot the PRKR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
PRKR antibody used in western blot to detect PRKR/PKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (2) . Predicted molecular weight ~62 kDa but routinely observed at 68-72 kDa.

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