NCAPH2 Antibody

• Description:

  Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q6IBW4

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 198-227 from the human protein was used as the immunogen for this NCAPH2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the NCAPH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This NCAPH2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the NCAPH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the NCAPH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of NCAPH2 antibody and HepG2 lysate.