ATF4 Antibody

• Description:

  ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2) . ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q96AQ3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 220-252 from the human protein was used as the immunogen for this ATF4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the ATF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This ATF4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the ATF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the ATF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of ATF4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (2) .