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Recombinant Mouse Granulocyte-macrophage colony-stimulating factor (Csf2) (Active)

CAT:
399-CSB-AP003651MO-02
Size:
500 µg
Price:
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For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Recombinant Mouse Granulocyte-macrophage colony-stimulating factor (Csf2) (Active) - image 1
Recombinant Mouse Granulocyte-macrophage colony-stimulating factor (Csf2) (Active) - image 2
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Recombinant Mouse Granulocyte-macrophage colony-stimulating factor (Csf2) (Active)

  • CAS Number: 9000-83-3
  • Gene Name: Csf2
  • UniProt: P01587
  • Expression Region: 18-141aa
  • Organism: Mus musculus
  • Target Sequence: APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPGQK
  • Tag: C-terminal 6xHis-tagged
  • Source: Mammalian cell
  • Field of Research: Immunology
  • Assay Type: Active Protein & In Stock Protein
  • Relevance: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
  • Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
  • Purity: Greater than 95% as determined by SDS-PAGE.
  • Activity: Yes
  • Bioactivity: The ED50 as determined in a cell proliferation assay using PDC-P1 cells is 40-170 pg/ml.
  • Length: Full Length of Mature Protein
  • Form: Lyophilized powder
  • Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4
  • Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Molecular Weight: 15.1 kDa
  • Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.