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WNT3A Antibody

CAT: 0013-GTR18225994Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225994Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
WNT3A Antibody
Product Name Alternative
WNT3A, Protein Wnt-3a
Reactivity
Human
Immunogen
E.coli derived recombinant Human WNT-3a.
Target
WNT3A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Immunoaffinity purified
Dilution
ELISA (0.5 - 2 μg/ml), IHC, IHC-P (5 μg/ml), WB (0.1 - 0.2 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Resuspension: Reconstitute with sterile water to 0.1-1.0 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. ELISA: To detect hWNT-3a by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
Lyophilized from PBS, pH 7.2

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