FGF2/Basic FGF Antibody

FGF2/Basic FGF Antibody

FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2

Human

E.coli derived recombinant Human FGF-basic.

FGF2 / Basic FGF

Unconjugated

Neuroscience; Cardiovascular Research; Stem Cell & Developmental Biology

Immunoaffinity purified

ELISA (0.5 - 2 μg/ml), IHC, IHC-P (5 μg/ml), WB (0.1 - 0.2 μg/ml)

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

Resuspension: Reconstitute in sterile water to 0.1-1 mg/ml. . Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/ml), a concentration of 0.25-0.40 ug/ml of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

ELISA, IHC, IHC-P, WB

Rabbit

Lyophilized from PBS, pH 7.2