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IGF1 Antibody

CAT: 0013-GTR18225899Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225899Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IGF1 Antibody
Product Name Alternative
IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF
Reactivity
Human
Immunogen
E.coli derived recombinant Human IGF-I.
Target
IGF1
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Immunoaffinity purified
Dilution
ELISA (0.5 - 2 μg/ml), IHC-P (5 μg/ml), WB (0.1 - 0.2 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Resuspension: Reconstitute with sterile water. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-I (5 ng/ml), a concentration of 0.67-1 ug/ml of this antibody is required. ELISA: To detect hIGF-I by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, IHC-P, WB
Host or Source
Rabbit
Preservative
Lyophilized from PBS, pH 7.2
Isotype
IgG

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