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HLA-DQA2 Antibody

CAT: 0013-GTR18225896Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225896Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HLA-DQA2 Antibody
Product Name Alternative
HLA-DQA2, DX-ALPHA, DX alpha chain, MHC class II DQA2, HLA-DXA
Reactivity
Bovine, Goat, Human, Sheep
Immunogen
Peptide with sequence C-QSHGPSGQYTH, from the internal region of the protein sequence according to NP_064440.1.
Target
HLA-DQA2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (3.75 μg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 35kD and 24kD in Human Lymph Node and Tonsil lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 28.0kD according to NP_064440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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