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TRPM2 Antibody

CAT: 0013-GTR18225386Size: 50 μLDry Ice: NoHazardous: No
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CAT#:0013-GTR18225386Size:50 μL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TRPM2 Antibody
Product Name Alternative
TRPM2, EREG1, LTrpC-2, LTRPC2, NUDT9L1, KNP3, NUDT9H, Trrp7
Reactivity
Human
Immunogen
Synthetic peptide located between aa431-480 of human TRPM2 (O94759, NP_003298) . Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%) ; Galago, Marmoset, Elephant, Bovine, Bat, Horse (92%) ; Guinea pig (87%) ; Panda, Platypus, Lizard (85%) ; Turkey, Zebra finch, Chicken (84%) .
Target
TRPM2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Immunoaffinity purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (10 - 20 μg/ml), WB (1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1μg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
PBS, 0.09% sodium azide, 2% sucrose
Isotype
IgG

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